Part:BBa_K4131000:Design

AccD1 gene (C. glutamicum), codon optimized for E. Coli expression

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 280
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The sequence is codon optimized for E.Coli expression, and the restriction enzyme sites of XbaI, SpeI, EcoRI, PstI, and NotI are eliminated.

Source

The sequence is extracted and modified from the region 1509844-1511475 in Corynebacterium glutamicum genome (CP025534.1)